Cellist Lukáš Pospíšil studied at the Prague Conservatory, the Academy of Performing Arts in Prague, the Hochschule für Musik und darstellende Kunst in Vienna. During his studies, he also attended several masterclasses (Heinrich Schiff, Martin Hornstein) and appeared at competitions (Beethoven’s Hradec, the Janáček International Music Competition in Brno).
From 1988 to 1991, he was a member and then later concertmaster of the Gustav Mahler Jugendorchester, and from 2004—2005 he was the solo cellist in the Kammerorchester Berlin. Also, Lukáš Pospíšil is a recognized chamber musician (Zeiher Quartett), and he has appeared regularly as a soloist in the Czech Republic as well as abroad. He is an enthusiastic promoter of works by Czech composers and together with the Czech Philharmonic premiered the Concerto for Cello and Orchestra by Zdeněk Šesták.
He has been the concertmaster of the PKF — Prague Philharmonia since 1994 with which he as the soloist recorded a CD conducted by Jiří Bělohlávek in 2000.